Journal: Hematology, Transfusion and Cell Therapy

Article Title: NT157 exhibits antineoplastic effects by targeting IRS and STAT3/5 signaling in multiple myeloma

doi: 10.1016/j.htct.2024.02.017

Figure Lengend Snippet: Differential expression of IGF1R signaling-related genes in multiple myeloma patients. (A) IGF1, IGF1R, IRS1 , and IRS2 mRNA levels in samples from healthy donors and multiple myeloma (MM) patients. The y-axis represents gene expression data obtained from the AmaZonia! database 2008, which were measured using Affymetrix HGU133 plus 2.0 arrays. The data sets were cross-referenced using tumor-specific identification numbers. The numbers of subjects for each group are indicated. The p -values are also indicated; Mann–Whitney U test. (B) Correlogram of IGF1R signaling-related genes in MM patients. The correlation analysis was performed using the Spearman test and RStudio software and the Corrplot plugin. The sizes of the circle indicate the r values and the color indicates the direction of the correlation (blue: positive correlation, red: negative correlation). (C) Western blot analysis p-IGF1R, IGF1R, IRS1, and IRS2 in the total extract from MM cell lines. The membranes were re-probed for the detection of α-tubulin and developed using a SuperSignal™ West Dura Extended Duration Substrate system and a G:BOX Chemi XX6 gel doc system. (D) Gene dependency scores for IGF1, IGF1R, IRS1 , and IRS2 of 20 MM cell lines were obtained from the MyeloDB database. For gene effect, a score less than -0.5 represents depletion in most cell lines, while less than -1 represents strong killing. (E) Representation of the NT157 chemical structure. CAS, Chemical Abstracts Service.

Article Snippet: The correlation analysis was performed using the Spearman test and RStudio software and the Corrplot plugin.

Techniques: Quantitative Proteomics, Gene Expression, MANN-WHITNEY, Software, Western Blot